Genetic investigation of human plasma lipidome to get. Specific changes in the lipidome suggested contributions from extracellular vesicles, viremia, liver dysfunction, apoptosis. Considering that the human plasma lipidome contains at least 600 named and quantifiable lipid species 45 but probably many more 46, our assays captured only a small part of the. Trapped ion mobility spectrometry and pasef enable in. Comprehensive lipidomics workflow using automated flow. Msbased lipidomics of human blood plasma the journal of lipid. The lipidome of the yeast saccharomyces cerevisiae has been characterised with an estimated 95% coverage. The human plasma proteome holds the promise of a revolution in disease diagnosis and therapeutic monitoring provided that major challenges in proteomics and related disciplines. Genetic architecture of human plasma lipidome and its link to. Plasmanyl and plasmenyl glycerophosphocholine are ether lipids featuring the 1oalkyl or 1oalk1. Comparison of rpc columns with different stationary phases. Disturbances in the plasma lipidome also occur in conditions that are not directly linked. Herein, we have investigated the plasma lipidome during the second half of the human lifespan, including a cohort of subjects aged 95 and above enrolled in the scs. Free radical biology and medicine 59 20 6984 open access under cc by.
We present a highthroughput, nontargeted lipidomics approach using liquid chromatography coupled to highresolution mass spectrometry for quantitative analysis of. Human blood edta plasma was obtained from healthy volunteers through venipuncture. Genetic correlation of the plasma lipidome with type 2. Consequently, the plasma lipidome has a considerable impact on the cellular lipid environment, vasculature function, and inflammatory and oxidative processes. To further show the efficacy of this approach, we devised a simple routine for extensive plasma lipidome characterization using only 8.
Multiplatformomicsanalysisofhumanebolavirus disease. The first cell lipidome to be published was that of a mouse macrophage in 2010. Most plasma metabolites and lipids were unaffected by storage time and. Bacs and metabolites can be quantified by lc msms and. Large scale lipid profiling of a human serum lipidome. Human blood is a selfregenerating lipidrich biological fluid that is routinely collected in hospital settings. Using liquid chromatography tandem mass spectrometry, we profiled the human plasma lipidome of patients that survived and succumbed to evd during the 2014 outbreak in sierra leone.
The likelihood of future outbreaks of similar or greater. Human plasma definition of human plasma by the free. Whether the wide interindividual variability in the. The results highlight the profound impact of evd on the host lipidome, leading to possible therapies for improving evd survival. Sample preparation plasma lipids were extracted by adding 200 l of icecold. Biochemical analyses reveal that plasma contains over 600 structural forms of lipid molecules. Differential plasma concentrations of circulating lipid species are associated with pathogenesis of type 2 diabetes t2d. Improving coverage of the plasma lipidome using iterative msms. Here we report a chromatography gradient coupled with a detailed characterization of the human plasma lipidome to provide improved resolution and identification of 636 lipid species, including previously unreported species, in a 15min analysis. The focus of the present study was to define the human plasma lipidome and to establish novel analytical methodologies to quantify the large spectrum of plasma lipids.
Traml is a file format for the exchange of targeted msms transition lists. Heterogeneity of lipidomic profiles among lung cancer. Metabolites free fulltext impact of blood collection. The mass spectrometry raw files were analyzed with metaboscape alpha version. The supplementary material 5 is a pdf file for lipidomics showing base peak ion.
Meanwhile, the human plasma lipidome, consisting of over 600 distinct molecular species, is correlated with certain clinical outcomes. Human plasma lipidome has been extensively studied in many pathophysiological contexts with the hope of identifying biomarkers for early diagnostics and monitoring the progression and treatment of a broad spectrum of diseases. Lipidomics is the largescale study of pathways and networks of cellular lipids in biological systems the word lipidome is used to describe the complete lipid profile within a cell, tissue. The inventory of lipid molecules found in blood plasma plasma.
Human blood plasma lipidome analyzed by reversephase lcms. Sample preparation nist srm 1950 plasma was thawed on. Easy, fast, and reproducible quantification of cholesterol. Genetic architecture of human plasma lipidome and its link. Changes in plasma lipidome following initiation of. Human plasma synonyms, human plasma pronunciation, human plasma translation, english dictionary definition of human plasma. The human proteome organization in 2003 launched an effort to combine results from the many labs around the world who were working on the human plasma proteome. Bacs can be metabolized by human cytochromes p 450. The raw data in mzdata format files exported from the masshunter software were imported into simlipid software. The effect of anticoagulants, temperature, and time on the human. From the departments of medicine and pharmacology o.
Plasma lipidome reveals critical illness and recovery from. Plasma lipidome variation during the second half of the. Machine learning of human plasma lipidomes for obesity estimation. Pdf genetic architecture of human plasma lipidome and. Profiling the human plasma lipidome provides insight into critical illness as well as diseased. This study examines the effects of experimentally induced shortterm and longterm gc excess induced by prednisolone and tetracosactide, respectively on the plasma lipidome of beale. In this study, we conducted a pilot analysis of serum and plasma to determine the effects of processing time and collection tube on the metabolome. Introduction hiv infection and the use of antiretroviral. In an effort to disentangle the human plasma lipidome. Human plasma proteome project data central at peptideatlas.
Understanding genetic architecture of plasma lipidome could provide better insights into lipid metabolism and its link to cardiovascular diseases cvds. Lipoproteins are responsible for the transport of lipids and other nutrients in the circulation and therefore play an important role in lipid metabolism and dyslipidemia. Analysis of ether glycerophosphocholines at the level of c. Nist srm 1950 human plasma was purchased from millipore sigma. This msbased automated shotgun lipidomics platform for comprehensive analysis of the blood plasma lipidome covering 22 lipid classes achieves high interday and intersite. Mass spectrometrybased lipidomics of human blood plasma 2003 methods unless wellharmonized, and we see no conceptual reason why data concordance could not be reached for. Identification of key lipids critical for platelet. Human plasma comprises nucleic acids, amino acids mostly in the form of proteins, carbohydrates in the form of monosaccharides and disaccharides, and lipids fig. Improving coverage of the plasma lipidome using iterative. How does metabolomics provide information on the circulating chemicals. The data presented in the figures and other summary level data are contained within the supplementary files and supplementary data. Highthroughput quantitative lipidomics analysis of. Largescale lipid profiling of a human serum lipidome using a highresolution, accuratemass lcmsms approach reiko kiyonami 1, david peake, devin drew 1, xuefei sun 2, xiaodong liu. Genetic regulation of plasma lipid species and their.
Rational selection of stationary phase based on the lipidome. Plasma lipidome reveals critical illness and recovery from human. The effect of anticoagulants, temperature, and time on the human plasma metabolome and lipidome from healthy donors as determined by liquid chromatographymass spectrometry. Human plasma lipidome has been extensively studied in many pathophysiological contexts with the hope of identifying biomarkers for early diagnostics and monitoring the progression and. Bioanalysis for precision medicine ehealth initiative. Human blood is a selfregenerating lipidrich biological fluid that is routinely. Aims we tested whether characteristic changes of the plasma lipidome in individuals with comparable total lipids level associate with future cardiovascular disease cvd outcome and.
Conclusions initiation of art lead to significant changes in the plasma lipidome that were greatest in those receiving efavirenz. Human blood is a selfregenerating, lipidrich biologic fluid that is routinely collected in hospital settings. The inventory of lipid molecules found in blood plasma plasma lipidome offers. Genetic architecture of human plasma lipidome and its. Genetic architecture of human plasma lipidome and its link to cardiovascular disease article pdf available in nature communications 101. Rational selection of reverse phase columns for high. Largescale lipid profiling of a human serum lipidome.
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